Composite

Part:BBa_K5136225:Design

Designed by: Xiaoxiao Zhang   Group: iGEM24_XMU-China   (2024-09-14)


I0500-B0034-mnta-B0015


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 3247
    Illegal SpeI site found at 3304
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 2203
    Illegal NheI site found at 3388
    Illegal SpeI site found at 3247
    Illegal SpeI site found at 3304
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 3247
    Illegal SpeI site found at 3304
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3247
    Illegal SpeI site found at 3304
    Illegal NgoMIV site found at 2039
    Illegal NgoMIV site found at 3408
    Illegal NgoMIV site found at 3481
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1672
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

1


Source

synthesis

References